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Plant and Fungal Diversity in Gut Microbiota as Revealed by Molecular and Culture Investigations
- Source :
- PLoS ONE, PLoS ONE, 2013, 8 (3), pp.e59474. ⟨10.1371/journal.pone.0059474⟩, PLoS ONE, Public Library of Science, 2013, 8 (3), pp.e59474. ⟨10.1371/journal.pone.0059474⟩, PLoS ONE, Vol 8, Iss 3, p e59474 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- International audience; Background: Few studies describing eukaryotic communities in the human gut microbiota have been published. The objective of this study was to investigate comprehensively the repertoire of plant and fungal species in the gut microbiota of an obese patient.Methodology/Principal Findings: A stool specimen was collected from a 27-year-old Caucasian woman with a body mass index of 48.9 who was living in Marseille, France. Plant and fungal species were identified using a PCR-based method incorporating 25 primer pairs specific for each eukaryotic phylum and universal eukaryotic primers targeting 18S rRNA, internal transcribed spacer (ITS) and a chloroplast gene. The PCR products amplified using these primers were cloned and sequenced. Three different culture media were used to isolate fungi, and these cultured fungi were further identified by ITS sequencing. A total of 37 eukaryotic species were identified, including a Diatoms (Blastocystis sp.) species, 18 plant species from the Streptophyta phylum and 18 fungal species from the Ascomycota, Basidiomycota and Chytridiocomycota phyla. Cultures yielded 16 fungal species, while PCR-sequencing identified 7 fungal species. Of these 7 species of fungi, 5 were also identified by culture. Twenty-one eukaryotic species were discovered for the first time in human gut microbiota, including 8 fungi (Aspergillus flavipes, Beauveria bassiana, Isaria farinosa, Penicillium brevicompactum, Penicillium dipodomyicola, Penicillium camemberti, Climacocystis sp. and Malassezia restricta). Many fungal species apparently originated from food, as did 11 plant species. However, four plant species (Atractylodes japonica, Fibraurea tinctoria, Angelica anomala, Mitella nuda) are used as medicinal plants.Conclusions/Significance: Investigating the eukaryotic components of gut microbiota may help us to understand their role in human health.
- Subjects :
- Nicotiana
Adult
Clinical Pathology
lcsh:Medicine
Mycology
Plant Science
Microbiology
Polymerase Chain Reaction
Feces
Medicinal plants
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Diagnostic Medicine
Culture Techniques
Pathology
Humans
Obesity
Gut bacteria
lcsh:Science
Biology
lcsh:R
fungi
Botany
Fungi
Eukaryota
Biodiversity
Plants
Intestines
Clinical Microbiology
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Ribosomal RNA
Medical Microbiology
Fungal Classification
Medicine
Metagenome
lcsh:Q
Female
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.pmid.dedup....46b8e25514f49bf00b651dce96f6eb5a