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Measurement of carbon flux through the MEP pathway for isoprenoid synthesis by (31)P-NMR spectroscopy after specific inhibition of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate reductase. Effect of light and temperature
- Source :
- Plant, Cell and Environment, Plant, Cell and Environment, 2011, 34 (8), pp.1241-7. ⟨10.1111/j.1365-3040.2011.02322.x⟩, Plant, Cell and Environment, Wiley, 2011, 34 (8), pp.1241-7. ⟨10.1111/j.1365-3040.2011.02322.x⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; The methylerythritol 4-phosphate (MEP) and the mevalonate pathways are the unique synthesis routes for the precursors of all isoprenoids. An original mean to measure the carbon flux through the MEP pathway in plants is proposed by using cadmium as a total short-term inhibitor of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (MEcDP) reductase (GcpE) and measuring the accumulation rate of its substrate MEcDP by (31) P-NMR spectroscopy. The MEP pathway metabolic flux was determined in spinach (Spinacia oleracea), pea (Pisum sativum), Oregon grape (Mahonia aquifolium) and boxwood (Buxus sempervirens) leaves. In spinach, flux values were compared with the synthesis rate of major isoprenoids. The flux increases with light intensity (fourfold in the 200-1200 µmol m(-2) s(-1) PPFR range) and temperature (sevenfold in the 25-37 °C range). The relationship with the light and the temperature dependency of isoprenoid production downstream of the MEP pathway is discussed.
- Subjects :
- Magnetic Resonance Spectroscopy
Buxus sempervirens
methylerythritol 4-phosphate
Mahonia
Mevalonic Acid
plant
Mahonia aquifolium
Carbon Cycle
isoprenoid biosynthesis
mevalonate
Spinacia oleracea
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Enzyme Inhibitors
Buxus
Pisum sativum
Terpenes
Peas
temperature
metabolic pathway
Plants
metabolic flux
nuclear magnetic resonance
Erythritol
Oxidoreductases
light
Phosphorus Radioisotopes
metabolism
Metabolic Networks and Pathways
Cadmium
Subjects
Details
- Language :
- English
- ISSN :
- 01407791 and 13653040
- Database :
- OpenAIRE
- Journal :
- Plant, Cell and Environment, Plant, Cell and Environment, 2011, 34 (8), pp.1241-7. ⟨10.1111/j.1365-3040.2011.02322.x⟩, Plant, Cell and Environment, Wiley, 2011, 34 (8), pp.1241-7. ⟨10.1111/j.1365-3040.2011.02322.x⟩
- Accession number :
- edsair.pmid.dedup....509a76c3fa4e10d9cf907f6a4e1a3a52