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Deoxynivalenol induces structural alterations in epidermoid carcinoma cells A431 and impairs the response to biomechanical stimulation

Deoxynivalenol induces structural alterations in epidermoid carcinoma cells A431 and impairs the response to biomechanical stimulation

Authors :
Giorgia Del Favero
Lydia Woelflingseder
Lukas Janker
Benjamin Neuditschko
Stefano Seriani
Paolo Gallina
Orfeo Sbaizero
Christopher Gerner
Doris Marko
Del Favero, Giorgia
Woelflingseder, Lydia
Janker, Luka
Neuditschko, Benjamin
Seriani, Stefano
Gallina, Paolo
Sbaizero, Orfeo
Gerner, Christopher
Marko, Doris
Source :
Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-17 (2018)
Publication Year :
2017

Abstract

Morphology together with the capability to respond to surrounding stimuli are key elements governing the spatial interaction of living cells with the environment. In this respect, biomechanical stimulation can trigger significant physiological cascades that can potentially modulate toxicity. Deoxynivalenol (DON, vomitoxin) is one of the most prevalent mycotoxins produced by Fusarium spp. and it was used to explore the delicate interaction between biomechanical stimulation and cytotoxicity in A431 cells. In fact, in addition of being a food contaminant, DON is a relevant toxin for several organ systems. The combination between biomechanical stimulation and the mycotoxin revealed how DON can impair crucial functions affecting cellular morphology, tubulin and lysosomes at concentrations even below those known to be cytotoxic in routine toxicity studies. Sub-toxic concentrations of DON (0.1–1 μM) impaired the capability of A431 cells to respond to a biomechanical stimulation that normally sustains trophic effects in these cells. Moreover, the effects of DON (0.1–10 μM) were partially modulated by the application of uniaxial stretching (0.5 Hz, 24 h, 15% deformation). Ultimately, proteomic analysis revealed the potential of DON to alter several proteins necessary for cell adhesion and cytoskeletal modulation suggesting a molecular link between biomechanics and the cytotoxic potential of the mycotoxin.

Details

ISSN :
20452322
Volume :
8
Issue :
1
Database :
OpenAIRE
Journal :
Scientific reports
Accession number :
edsair.pmid.dedup....55ea224753dcdf3b48e3ec1bdc8a2655