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Distinct responses to ozone of abaxial and adaxial stomata in three Euramerican poplar genotypes

Authors :
Dumont, Jennifer
Cohen, David
Gérard, Joëlle
Jolivet, Yves
Dizengremel, Pierre
Le Thiec, Didier
Ecosystèmes Forestiers, Agroressources, Bioprocédés et Alimentation (EFABA)
Université de Lorraine (UL)
Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF)
Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL)
French National Research Agency, ANR, project 'Vulnoz'
ANR
Lorraine Region
French National Research Agency through the Laboratory of Excellence ARBRE [ANR-12-LABXARBRE-01]
[IFR 110 EFABA]
Source :
Plant, Cell and Environment, Plant, Cell and Environment, Wiley, 2014, 37 (9), pp.2064-2076. ⟨10.1111/pce.12293⟩
Publication Year :
2014
Publisher :
HAL CCSD, 2014.

Abstract

Ozone induces stomatal sluggishness, which impacts photosynthesis and transpiration. Stomatal responses to variation of environmental parameters are slowed and reduced by ozone and may be linked to difference of ozone sensitivity. Here we determine the ozone effects on stomatal conductance of each leaf surface. Potential causes of this sluggish movement, such as ultrastructural or ionic fluxes modification, were studied independently on both leaf surfaces of three Euramerican poplar genotypes differing in ozone sensitivity and in stomatal behaviour. The element contents in guard cells were linked to the gene expression of ion channels and transporters involved in stomatal movements, directly in microdissected stomata. In response to ozone, we found a decrease in the stomatal conductance of the leaf adaxial surface correlated with high calcium content in guard cells compared with a slight decrease on the abaxial surface. No ultrastructural modifications of stomata were shown except an increase in the number of mitochondria. The expression of vacuolar H(+) /Ca(2+) -antiports (CAX1 and CAX3 homologs), β-carbonic anhydrases (βCA1 and βCA4) and proton H(+) -ATPase (AHA11) genes was strongly decreased under ozone treatment. The sensitive genotype characterized by constitutive slow stomatal response was also characterized by constitutive low expression of genes encoding vacuolar H(+) /Ca(2+) -antiports.

Details

Language :
English
ISSN :
01407791 and 13653040
Database :
OpenAIRE
Journal :
Plant, Cell and Environment, Plant, Cell and Environment, Wiley, 2014, 37 (9), pp.2064-2076. ⟨10.1111/pce.12293⟩
Accession number :
edsair.pmid.dedup....5c760e890e540f6b5e2822400f261f5e
Full Text :
https://doi.org/10.1111/pce.12293⟩