Back to Search
Start Over
Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody
- Source :
- Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018), Nature Communications, 9, 1, pp. 3822, Nature Communications, 9, 3822
- Publication Year :
- 2018
- Publisher :
- Nature Publishing Group, 2018.
-
Abstract
- Contains fulltext : 201378.pdf (Publisher’s version ) (Open Access) The quest to develop an effective malaria vaccine remains a major priority in the fight against global infectious disease. An approach with great potential is a transmission-blocking vaccine which induces antibodies that prevent establishment of a productive infection in mosquitos that feed on infected humans, thereby stopping the transmission cycle. One of the most promising targets for such a vaccine is the gamete surface protein, Pfs48/45. Here we establish a system for production of full-length Pfs48/45 and use this to raise a panel of monoclonal antibodies. We map the binding regions of these antibodies on Pfs48/45 and correlate the location of their epitopes with their transmission-blocking activity. Finally, we present the structure of the C-terminal domain of Pfs48/45 bound to the most potent transmission-blocking antibody, and provide key molecular information for future structure-guided immunogen design.
- Subjects :
- Membrane Glycoproteins
Science
Protozoan Proteins
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
Antibodies, Monoclonal
Malaria
Epitopes
Mice
All institutes and research themes of the Radboud University Medical Center
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Protein Domains
Malaria Vaccines
Protein Interaction Mapping
Animals
Immunization
lcsh:Q
Antibodies, Blocking
lcsh:Science
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.pmid.dedup....5feae0f58b913d189c2bcdf1a55eafe1