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Putative FLJ20436 gene characterisation in goat. Observed ubiquitous expression in goat and transgenic mice allowed to restrict the location of an hypothesised insulator element

Authors :
Mata, Xavier
Taourit, Sead
Le Provost, Fabienne
Unité de recherche Génétique Biochimique et Cytogénétique (LGBC)
Institut National de la Recherche Agronomique (INRA)
Génétique Animale et Biologie Intégrative (GABI)
Institut National de la Recherche Agronomique (INRA)-AgroParisTech
Source :
Gene, Gene, Elsevier, 2003, 321, pp.137-144, Gene, Elsevier, 2003, 321, pp.137-44
Publication Year :
2003
Publisher :
HAL CCSD, 2003.

Abstract

International audience; Eukaryotic genomes are organised into independent domains through the establishment of boundaries which allow to have distinct pattern of gene expression both during development and in differentiated cells. The previously reported site independent expression of the mammary-specific goat alpha-lactalbumin gene in transgenic mice suggested the existence of cis-regulatory elements located upstream of this gene. The nearby presence of a second ubiquitously expressed gene (the cyclin T1 gene) allowed to define two chromatin domains putatively separated by a boundary insulator-like element. In this study, the characterisation of a third putative gene (FLJ20436) present between the alpha-lactalbumin and the cyclin T1 loci is reported. It was found to be a functional gene, ubiquitously expressed both in goat and transgenic mice. A complex pattern of alternative splicing events was observed in several analysed tissues leading to various mRNAs and putative FLJ20436 proteins, as suggested in human and mouse species. This allowed us to assign this gene to one of the two hypothesised chromatin domains, refining the location of the potential insulator element.

Details

Language :
English
ISSN :
03781119 and 18790038
Database :
OpenAIRE
Journal :
Gene, Gene, Elsevier, 2003, 321, pp.137-144, Gene, Elsevier, 2003, 321, pp.137-44
Accession number :
edsair.pmid.dedup....62050ab94270d1863beca8d91a4d5b47