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Putative FLJ20436 gene characterisation in goat. Observed ubiquitous expression in goat and transgenic mice allowed to restrict the location of an hypothesised insulator element
- Source :
- Gene, Gene, Elsevier, 2003, 321, pp.137-144, Gene, Elsevier, 2003, 321, pp.137-44
- Publication Year :
- 2003
- Publisher :
- HAL CCSD, 2003.
-
Abstract
- International audience; Eukaryotic genomes are organised into independent domains through the establishment of boundaries which allow to have distinct pattern of gene expression both during development and in differentiated cells. The previously reported site independent expression of the mammary-specific goat alpha-lactalbumin gene in transgenic mice suggested the existence of cis-regulatory elements located upstream of this gene. The nearby presence of a second ubiquitously expressed gene (the cyclin T1 gene) allowed to define two chromatin domains putatively separated by a boundary insulator-like element. In this study, the characterisation of a third putative gene (FLJ20436) present between the alpha-lactalbumin and the cyclin T1 loci is reported. It was found to be a functional gene, ubiquitously expressed both in goat and transgenic mice. A complex pattern of alternative splicing events was observed in several analysed tissues leading to various mRNAs and putative FLJ20436 proteins, as suggested in human and mouse species. This allowed us to assign this gene to one of the two hypothesised chromatin domains, refining the location of the potential insulator element.
- Subjects :
- Male
GENE EXPRESSION
MESH: Gene Expression
Transcription, Genetic
MESH: Mice, Transgenic
MESH: Introns
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Mice, Transgenic
Response Elements
MESH: Goats
STRUCTURE DES GENES
GOATS
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
MESH: Promoter Regions, Genetic
Animals
Humans
MESH: Animals
Promoter Regions, Genetic
GENE STRUCTURE
MESH: Mice
Conserved Sequence
MESH: Molecular Sequence Data
MESH: Conserved Sequence
MESH: Humans
MESH: Alternative Splicing
MESH: Transcription, Genetic
Chromosome Mapping
Exons
Introns
MESH: Male
MESH: Genes
Alternative Splicing
MICE
Genes
MESH: Response Elements
MESH: Transcription Initiation Site
Transcription Initiation Site
MESH: Poly A
SPLICING
Poly A
MESH: Chromosome Mapping
MESH: Exons
Subjects
Details
- Language :
- English
- ISSN :
- 03781119 and 18790038
- Database :
- OpenAIRE
- Journal :
- Gene, Gene, Elsevier, 2003, 321, pp.137-144, Gene, Elsevier, 2003, 321, pp.137-44
- Accession number :
- edsair.pmid.dedup....62050ab94270d1863beca8d91a4d5b47