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Dissociation between skin test reactivity and anti-aeroallergen IgE: Determinants among urban Brazilian children
- Source :
- PLoS ONE, Vol 12, Iss 3, p e0174089 (2017), PLoS ONE, 12(3), PLoS ONE
- Publication Year :
- 2017
-
Abstract
- BACKGROUND: The dissociation between specific IgE and skin prick test reactivity to aeroallergens, a common finding in populations living in low and middle-income countries, has important implications for the diagnosis and treatment of allergic diseases. Few studies have investigated the determinants of this dissociation. In the present study, we explored potential factors explaining this dissociation in children living in an urban area of Northeast Brazil, focusing in particular on factors associated with poor hygiene. METHODS: Of 1445 children from low income communities, investigated for risk factors of allergies, we studied 481 with specific IgE antibodies to any of Blomia tropicalis, Dermatophagoides pteronyssinus, Periplaneta americana and Blatella germanica allergens. Data on demographic, environmental and social exposures were collected by questionnaire; serum IgG and stool examinations were done to detect current or past infections with viral, bacterial, protozoan and intestinal helminth pathogens. We measured atopy by skin prick testing (SPT) and specific IgE (sIgE) to aerollergens in serum (by ImmunoCAP). SIgE reactivity to B. tropicalis extract depleted of carbohydrates was measured by an in-house ELISA. Total IgE was measured by in house capture ELISA. SNPs were typed using Illumina Omni 2.5. RESULTS: Negative skin prick tests in the presence of specific IgE antibodies were frequent. Factors independently associated with a reduced frequency of positive skin prick tests were large number of siblings, the presence of IgG to herpes simplex virus, Ascaris lumbricoides and Trichuris trichiura infections, living in neighborhoods with infrequent garbage collection, presence of rodents and cats in the household and sIgE reactivity to glycosylated B. tropicalis allergens. Also, SNP on IGHE (rs61737468) was negatively associated with SPT reactivity. CONCLUSIONS: A variety of factors were found to be associated with decreased frequency of SPT such as unhygienic living conditions, infections, total IgE, IgE response to glycosylated allergens and genetic polymorphisms, indicating that multiple mechanisms may be involved. Our data, showing that exposures to an unhygienic environment and childhood infections modulate immediate allergen skin test reactivity, provide support for the "hygiene hypothesis".
- Subjects :
- lcsh:Medicine
Families
Feces
Allergies
Medicine and Health Sciences
Simplexvirus
Enzyme-Linked Immunoassays
Child
lcsh:Science
Children
Pathology and laboratory medicine
Organic Compounds
Medical microbiology
Chemistry
Trichuris
Helminth Infections
Child, Preschool
Physical Sciences
Viruses
Pathogens
Brazil
Research Article
Herpesviruses
Immunology
Carbohydrates
Enzyme-Linked Immunosorbent Assay
Rodentia
Research and Analysis Methods
Microbiology
Polymorphism, Single Nucleotide
Diagnostic Medicine
Helminths
Parasitic Diseases
Hypersensitivity
Animals
Epstein-Barr virus
Humans
Immunoassays
Ascaris lumbricoides
Skin Tests
Receptors, IgE
Organic Chemistry
lcsh:R
Organisms
Chemical Compounds
Viral pathogens
Urban Health
Biology and Life Sciences
Allergens
Immunoglobulin E
Invertebrates
Microbial pathogens
Age Groups
Immunoglobulin G
People and Places
Immunologic Techniques
Cats
Immunoglobulin epsilon-Chains
Clinical Immunology
Population Groupings
lcsh:Q
Clinical Medicine
DNA viruses
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, Vol 12, Iss 3, p e0174089 (2017), PLoS ONE, 12(3), PLoS ONE
- Accession number :
- edsair.pmid.dedup....669d0fbb926d2f5f462b0060b8793df2