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B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2018, 13 (4), pp.e0196470. ⟨10.1371/journal.pone.0196470⟩, PLoS ONE, 2018, 13 (4), pp.e0196470. ⟨10.1371/journal.pone.0196470⟩, PLoS ONE, Vol 13, Iss 4, p e0196470 (2018), PLoS ONE, Public Library of Science, 2018, 13 (4), ⟨10.1371/journal.pone.0196470⟩
- Publication Year :
- 2017
-
Abstract
- International audience; BackgroundBetter understanding of the immune response directed against Mycobacterium tuberculosis (Mtb) is critical for development of vaccine strategies and diagnosis tests. Previous studies suggested that Mtb enzymes involved in lipid metabolism, are associated with persistence and/or reactivation of dormant bacilli.MethodsCirculating antibodies secreting cells (ASCs), memory B cells, and antibodies directed against Cut4 (Rv3452) and CFP21 (Rv1984c) antigens were explored in subjects with either active- or latent-tuberculosis (LTB), and in Mtb-uninfected individuals.ResultsCirculating anti-Cut4 ASCs were detected in 11/14 (78.6%) subjects from the active TB group vs. 4/17 (23.5%) from the LTB group (p = 0.001). Anti-CFP21 ASCs were found in 11/14 (78.6%) active TB vs. in 5/17 (29.4%) LTB cases (p = 0.01). Circulating anti-Cut4 and anti-CFP21 ASCs were not detected in 38 Mtb uninfected controls. Memory B cells directed against either Cut4 or CFP21 were identified in 8/11 (72.7%) and in 9/11 (81.8%) subjects with LTB infection, respectively, and in 2/6 Mtb uninfected individuals (33.3%). High level of anti-Cut4 and anti-CFP21 IgG were observed in active TB cases.ConclusionCirculating IgG SCs directed against Cut4 or CFP21 were mostly detected in patients presenting an active form of the disease, suggesting that TB reactivation triggers an immune response against these two antigens.
- Subjects :
- Male
Bacterial Diseases
B Cells
Physiology
health care facilities, manpower, and services
[SDV]Life Sciences [q-bio]
lcsh:Medicine
Biochemistry
White Blood Cells
Animal Cells
Immune Physiology
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
Immune Response
health care economics and organizations
B-Lymphocytes
Immune System Proteins
Middle Aged
Antibodies, Bacterial
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Enzymes
[SDV] Life Sciences [q-bio]
Actinobacteria
Infectious Diseases
BCG Vaccine
Female
Cellular Types
Research Article
Adult
Immune Cells
education
Immunology
Enzyme-Linked Immunosorbent Assay
Research and Analysis Methods
Antibodies
Bacterial Proteins
Latent Tuberculosis
Humans
Tuberculosis
Immunoassays
Antibody-Producing Cells
Aged
Antigens, Bacterial
Blood Cells
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Cell Biology
Lipid Metabolism
Tropical Diseases
Memory B cells
Immunoglobulin G
Leukocytes, Mononuclear
Immunologic Techniques
Enzymology
lcsh:Q
Tomography, X-Ray Computed
Carboxylic Ester Hydrolases
Immunologic Memory
Mycobacterium Tuberculosis
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.pmid.dedup....9e663d64ba4768e132af0b07bb086674