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Structural characterization of T-protein of the Escherichia coli glycine cleavage system by X-ray small angle scattering
- Source :
- EMBL-EBI
- Publication Year :
- 2004
-
Abstract
- T-protein, one of the components of the glycine cleavage complex, catalyses the formation of ammonia and methylene-tetrahydrofolate from H-protein-bound intermediate. Native T-protein of the glycine cleavage system from E. coli was efficiently purified using a combination of hydrophobic interaction, gel permeation and ion exchange chromatography. Synchrotron radiation small angle X-ray solution scattering indicates that T-protein has an extended structure in solution. A low resolution model of the protein was constructed ab initio and tentative models of the tertiary structure were built using prediction methods constrained by the scattering data.
Details
- ISSN :
- 01455680
- Database :
- OpenAIRE
- Journal :
- Cellular and molecular biology (Noisy-le-Grand, France)
- Accession number :
- edsair.pmid.dedup....a4ce8282f93863854ddb1b1c51119a47