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Towards automated cancer screening : label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy
- Source :
- Journal of Biophotonics, Woolford, L, Chen, M, Dholakia, K & Herrington, C S 2018, ' Towards automated cancer screening: label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy ', Journal of biophotonics, pp. e201700244 . https://doi.org/10.1002/jbio.201700244
- Publication Year :
- 2018
-
Abstract
- This work was supported by the Engineering and Physical Sciences Research Council and Medical Research Council [EP/L016559/1, EP/P030017/1], and CRUK [A18075 Core Award]. The ability to provide quantitative, objective and automated pathological analysis would provide enormous benefits for national cancer screening programmes, in terms of both resource reduction and improved patient wellbeing. The move towards molecular pathology through spectroscopic methods shows great promise, but has been restricted by spectral quality, acquisition times and lack of direct clinical application. In this paper, we present the application of wavelength modulated Raman spectroscopy for the automated label- and fluorescence-free classification of fixed squamous epithelial cells in suspension, such as those produced during a cervical smear test. Direct comparison with standard Raman spectroscopy shows marked improvement of sensitivity and specificity when considering both human papillomavirus (sensitivity +12.0%, specificity +5.3%) and transformation status (sensitivity +10.3%, specificity +11.1%). Studies on the impact of intracellular sampling location and storage effects suggest that wavelength modulated Raman spectroscopy is sufficiently robust to be used in fixed cell classification, but requires further investigations of potential sources of molecular variation in order to improve current clinical tools. Publisher PDF
- Subjects :
- RC0254 Neoplasms. Tumors. Oncology (including Cancer)
screening
Full Articles
Intracellular Space
NDAS
Epithelial Cells
Spectrum Analysis, Raman
T Technology
RC0254
Automation
QC Physics
Full Article
SDG 3 - Good Health and Well-being
Cell Line, Tumor
cervix
Raman spectroscopy
Screening
cancer
Humans
Mass Screening
Early Detection of Cancer
QC
Cancer
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Biophotonics, Woolford, L, Chen, M, Dholakia, K & Herrington, C S 2018, ' Towards automated cancer screening: label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy ', Journal of biophotonics, pp. e201700244 . https://doi.org/10.1002/jbio.201700244
- Accession number :
- edsair.pmid.dedup....b047a7a61f306504000025c3232493c5