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A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
- Source :
- Nature Communications, Nature Communications, Nature Publishing Group, 2018, 9, ⟨10.1038/s41467-018-05869-z⟩, Nature Communications (9), . (2018), Nature Communications, 2018, 9, ⟨10.1038/s41467-018-05869-z⟩, Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018), CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses. Fil: Peris, Leticia. Universite Grenoble Alpes; Francia Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universite Grenoble Alpes; Francia Fil: Martinez Hernandez, José. Universidad del País Vasco; España. Universite Grenoble Alpes; Francia Fil: Saoudi, Yasmina. Universite Grenoble Alpes; Francia Fil: Jonckheere, Julie. Universite Grenoble Alpes; Francia Fil: Rolland, Marta. Universite Grenoble Alpes; Francia Fil: Sebastien, Muriel. Universite Grenoble Alpes; Francia Fil: Brocard, Jacques. Universite Grenoble Alpes; Francia Fil: Denarier, Eric. Universite Grenoble Alpes; Francia Fil: Bosc, Christopher. Universite Grenoble Alpes; Francia Fil: Guerin, Christophe. Universite Grenoble Alpes; Francia. Inserm; Francia Fil: Gory Fauré, Sylvie. Universite Grenoble Alpes; Francia Fil: Deloulme, Jean Christophe. Universite Grenoble Alpes; Francia Fil: Lanté, Fabien. Universite Grenoble Alpes; Francia Fil: Arnal, Isabelle. Universite Grenoble Alpes; Francia Fil: Buisson, Alain. Universite Grenoble Alpes; Francia Fil: Goldberg, Yves. Universite Grenoble Alpes; Francia Fil: Blanchoin, Laurent. Inserm; Francia. Universite Grenoble Alpes; Francia Fil: Delphin, Christian. Universite Grenoble Alpes; Francia Fil: Andrieux, Annie. Universite Grenoble Alpes; Francia
- Subjects :
- Dendritic Spines
Science
[SDV]Life Sciences [q-bio]
Mice, Transgenic
macromolecular substances
Hippocampus
Microtubules
Ciencias Biológicas
purl.org/becyt/ford/1 [https]
actine
Biología Celular, Microbiología
Fluorescence Resonance Energy Transfer
Animals
Humans
lcsh:Science
purl.org/becyt/ford/1.6 [https]
protéine int 6
Cells, Cultured
Actin
Mice, Knockout
Neurons
Photobleaching
phosphorylation
Dendrites
Mice, Inbred C57BL
Actin Cytoskeleton
filament
MAP6
lcsh:Q
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Microtubule-Associated Proteins
actin
CIENCIAS NATURALES Y EXACTAS
microtubule
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Journal :
- Nature Communications, Nature Communications, Nature Publishing Group, 2018, 9, ⟨10.1038/s41467-018-05869-z⟩, Nature Communications (9), . (2018), Nature Communications, 2018, 9, ⟨10.1038/s41467-018-05869-z⟩, Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018), CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Accession number :
- edsair.pmid.dedup....b4dc5c451f8c604e49214318af4db920
- Full Text :
- https://doi.org/10.1038/s41467-018-05869-z⟩