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The proximal Gata4 promoter directs reporter gene expression to sertoli cells during mouse gonadal development
- Source :
- Biology of Reproduction, Biology of Reproduction, 2007, 76 (1), pp.85-95. ⟨10.1095/biolreprod.106.055137⟩, Biology of Reproduction, Society for the Study of Reproduction, 2007, 76 (1), pp.85-95. ⟨10.1095/biolreprod.106.055137⟩
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- International audience; The GATA4 transcription factor is an important developmental determinant for many organs, such as the heart, gut, and testis. Despite this pivotal role, our understanding of the transcriptional mechanisms that control the proper spatiotemporal expression of the GATA4 gene remains limited. We have generated transgenic mice expressing a green fluorescent protein (GFP) marker under the control of rat Gata4 5' flanking sequences. Several GATA4-expressing organs displayed GFP fluorescence, including the heart, intestine, and pancreas. In the gonads, while GATA4 is expressed in pregranulosa, granulosa, and theca ovarian cells, and Sertoli, Leydig, and peritubular testicular cells, the first 5 kb of Gata4 regulatory sequences immediately upstream of exon 1 were sufficient to direct GFP reporter expression only in testis and, specifically, in Sertoli cells. Onset of GFP expression occurred after Sertoli cell commitment and was maintained in these cells throughout development to adulthood. In vitro studies revealed that the first 118 bp of the Gata4 promoter is sufficient for full basal activity in several GATA4-expressing cell lines. Promoter mutagenesis and DNA-binding experiments identified two GC-box motifs and, particularly, one E-box element within this -118-bp region that are crucial for its activity. Further analysis revealed that members of the USF family of transcription factors, especially USF2, bind to and activate the Gata4 promoter via this critical E-box motif.
- Subjects :
- Male
endocrine system
MESH: Mice, Transgenic
Sertoli cells
Green Fluorescent Proteins
Molecular Sequence Data
Mice, Transgenic
MESH: Transgenes
testis
MESH: Base Sequence
MESH: E-Box Elements
MESH: GATA4 Transcription Factor
E-Box Elements
Mice
developmental biology
MESH: Green Fluorescent Proteins
MESH: Sertoli Cells
Genes, Reporter
MESH: Promoter Regions, Genetic
MESH: Gene Expression Regulation, Developmental
Animals
MESH: Animals
Transgenes
Promoter Regions, Genetic
MESH: Mice
Conserved Sequence
Sex Characteristics
MESH: Molecular Sequence Data
MESH: Conserved Sequence
Base Sequence
urogenital system
MESH: Testis
MESH: Genes, Reporter
Gene Expression Regulation, Developmental
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
MESH: Male
GATA4 Transcription Factor
cardiovascular system
gene regulation
MESH: Sex Characteristics
Subjects
Details
- Language :
- English
- ISSN :
- 00063363 and 15297268
- Database :
- OpenAIRE
- Journal :
- Biology of Reproduction, Biology of Reproduction, 2007, 76 (1), pp.85-95. ⟨10.1095/biolreprod.106.055137⟩, Biology of Reproduction, Society for the Study of Reproduction, 2007, 76 (1), pp.85-95. ⟨10.1095/biolreprod.106.055137⟩
- Accession number :
- edsair.pmid.dedup....b81fc47ccaa2edf64b2286e7eff5ace1