Back to Search
Start Over
Different levels of variability in subtypes 1b and 4a of hepatitis C viruses
- Source :
- Scopus-Elsevier, Europe PubMed Central
-
Abstract
- We performed genetic and phenic analyses to evaluate nucleotide and amino-acid sequences of the amino-terminus of the E1 protein of HCV genotype 1b (extracted from databank) and 4a (characterised in this study). The non-synonymous (ka) mutation analysis demonstrated that the genome of genotype 1b was not saturated by variations, with a rate of transition/transversion (s/v) of 1.5, which is similar to the expected ratio (i.e., 2.0). The s/v ratio in genotype 4a isolates was lower (0.98), indicating saturation due long-term variability. Moreover, the genotype 1b sequences showed a higher number of ka mutations (s+v) (mean of 2.8 per sequence) than genotype 4a (mean of 1.5). The introduction of ka mutations resulted in a higher degree of amino acid variability in genotype 4a. In the genome of genotype 1b, each nucleotide mutation introduced new amino acids, with a Granthan distance of 3.35-42.5, whereas for genotype 4a the distances ranged from 48.8 to 102.1. The phenic analysis also indicated different and complex patterns of amino-acid substitution. Finally, diverse isoelectric points and hydrophobicity were predicted for the two genotypes, with a higher acidity for genotype 4a E1 proteins.
- Subjects :
- DNA, Complementary
Genotype
DNA Mutational Analysis
Hepacivirus
Databases
Viral Envelope Proteins
Genetic
Complementary
Codon
Genetic Variation
Hydrophobic and Hydrophilic Interactions
Amino Acids
Humans
Isoelectric Point
Selection, Genetic
RNA, Viral
Databases, Nucleic Acid
Mutation
Amino Acid Substitution
Viral
Selection
Settore MED/12 - Gastroenterologia
Nucleic Acid
DNA
RNA
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier, Europe PubMed Central
- Accession number :
- edsair.pmid.dedup....c542a3db1c89b960da7f8699c61ff3b5