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Antibacterial and anti-inflammatory activity of a temporin B peptide analogue on an in vitro model of cystic fibrosis
- Publication Year :
- 2014
-
Abstract
- Natural peptides with antimicrobial properties are deeply investigated as tools to fight bacteria resistant to common antibiotics. Small peptides, as those belonging to the temporin family, are very attractive because their activity can easily be tuned after small modification to their primary sequence. Structure-activity studies previously reported by us allowed the identification of one peptide, analogue of temporin B, TB_KKG6A, showing, unlike temporin B, antimicrobial activity against both Gram-positive and Gram-negative bacteria. In this paper, we investigated the antimicrobial and anti-inflammatory activity of the peptide TB_KKG6A against Pseudomonas aeruginosa. Interestingly, we found that the peptide exhibits antimicrobial activity at low concentrations, being able to downregulate the pro-inflammatory chemokines and cytokines interleukin (IL)-8, IL-1β, IL-6 and tumor necrosis factor-α produced downstream infected human bronchial epithelial cells. Experiments were carried out also with temporin B, which was found to show pro-inflammatory activity. Details on the interaction between TB_KKG6A and the P. aeruginosa LPS were obtained by circular dichroism and fluorescence studies.
- Subjects :
- Lipopolysaccharides
temporin, antimicrobial, anti-inflammatory, Pseudomonas aeruginosa, pro-inflammatory, peptide
Cystic Fibrosis
Molecular Conformation
temporin
Bronchi
Microbial Sensitivity Tests
Respiratory Mucosa
Cell Line
NO
Humans
Pseudomonas Infections
anti-inflammatory
Pseudomonas aeruginosa, anti-inflammatory, antimicrobial, peptide, pro-inflammatory, temporin
Circular Dichroism
Anti-Inflammatory Agents, Non-Steroidal
Proteins
peptide
Anti-Bacterial Agents
pro-inflammatory
Spectrometry, Fluorescence
Gene Expression Regulation
Drug Design
Pseudomonas aeruginosa
antimicrobial
Inflammation Mediators
Antimicrobial Cationic Peptides
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid.dedup....c66bfbf2c6c9a9ee641602d46c1d4f3b