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Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2018, ⟨10.1371/journal.pone.0202531⟩, PLoS ONE, Vol 13, Iss 8, p e0202531 (2018), PLoS ONE, 2018, ⟨10.1371/journal.pone.0202531⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- International audience; Microcontact printing has become a versatile soft lithography technique used to produce molecular micro-and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and biomedical applications, the large-scale implementation of microcontact printing is still an issue. It is hindered by the stamp-inking step that is critical to ensure a reproducible and uniform transfer of inked molecules over large areas. This is particularly important when addressing application such as cell microarray manufacturing, which are currently used for a wide range of analytical and pharmaceutical applications. In this paper, we present a large-scale and multiplexed microcontact printing process of extra-cellular matrix proteins for the fabrication of cell microarrays. We have developed a micro-fluidic inking approach combined with a magnetic clamping technology that can be adapted to most standard substrates used in biology. We have demonstrated a significant improvement of homogeneity of printed protein patterns on surfaces larger than 1 cm 2 through the control of both the flow rate and the wetting mechanism of the stamp surface during micro-fluidic inking. Thanks to the reproducibility and integration capabilities provided by microflui-dics, we have achieved the printing of three different adhesion proteins in one-step transfer. Selective cell adhesion and cell shape adaptation on the produced patterns were observed, showing the suitability of this approach for producing on-demand large-scale cell microarrays.
- Subjects :
- [SDV.BIO]Life Sciences [q-bio]/Biotechnology
Imaging Techniques
Microarrays
Microfluidics
Materials Science
Equipment
lcsh:Medicine
Biosensing Techniques
Fluid Mechanics
Research and Analysis Methods
Continuum Mechanics
Fluorescence Imaging
Cell Adhesion
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
lcsh:Science
Cell Shape
Materials by Attribute
Flow Rate
Extracellular Matrix Proteins
Physics
lcsh:R
Classical Mechanics
Biology and Life Sciences
Fluid Dynamics
Laboratory Glassware
Cell Biology
Microfluidic Analytical Techniques
Laboratory Equipment
Bioassays and Physiological Analysis
Manufacturing Processes
Tissue Array Analysis
Physical Sciences
Magnets
Printing
Engineering and Technology
lcsh:Q
Fluidics
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Public Library of Science, 2018, ⟨10.1371/journal.pone.0202531⟩, PLoS ONE, Vol 13, Iss 8, p e0202531 (2018), PLoS ONE, 2018, ⟨10.1371/journal.pone.0202531⟩
- Accession number :
- edsair.pmid.dedup....cb637d110e81d4a7ce9eacdbc3658d9f