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MALDI-TOF MS identification of Anopheles gambiae Giles blood meal crushed on Whatman filter papers
- Source :
- PLoS ONE, PLoS ONE, 2017, 12 (8), pp.e0183238. ⟨10.1371/journal.pone.0183238⟩, PLoS ONE, Public Library of Science, 2017, 12 (8), pp.e0183238. ⟨10.1371/journal.pone.0183238⟩, PLoS ONE, Vol 12, Iss 8, p e0183238 (2017)
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- International audience; Background: Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens are crushed on Whatman filter papers to determine the host feeding patterns by identifying the origin of their blood meals. The aim of this study was to test whether crushing engorged mosquito abdomens on Whatman filter papers was compatible with MALDI-TOF MS for mosquito blood meal identification. Both laboratory reared and field collected mosquitoes were tested.Material and methods: Sixty Anopheles gambiae Giles were experimentally engorged on the blood of six distinct vertebrate hosts (human, sheep, rabbit, dog, chicken and rat). The engorged mosquito abdomens were crushed on Whatman filter papers for MALDI-TOF MS analysis. 150 Whatman filter papers, with mosquitoes engorged on cow and goat blood, were preserved. A total of 77 engorged mosquito abdomens collected in the Comoros Islands and crushed on Whatman filter papers were tested with MALDI-TOF MS. Results The MS profiles generated from mosquito engorged abdomens crushed on Whatman filter papers exhibited high reproducibility according to the original host blood. The blood meal host was correctly identified from mosquito abdomens crushed on Whatman filter papers by MALDI-TOF MS. The MS spectra obtained after storage were stable regardless of the room temperature and whether or not they were frozen. The MS profiles were reproducible for up to three months. For the Comoros samples, 70/77 quality MS spectra were obtained and matched with human blood spectra. This was confirmed by molecular tools.Conclusion: The results demonstrated that MALDI-TOF MS could identify mosquito blood meals from Whatman filter papers collected in the field during entomological surveys. The application of MALDI-TOF MS has proved to be rapid and successful, making it a new and efficient tool for mosquito-borne disease surveillance.
- Subjects :
- Physiology
lcsh:Medicine
Disease Vectors
Mosquitoes
Mass Spectrometry
Analytical Chemistry
Spectrum Analysis Techniques
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Abdomen
Medicine and Health Sciences
lcsh:Science
Body Fluids
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Insects
Laboratory Equipment
Chemistry
Infectious Diseases
Blood
Physical Sciences
Vertebrates
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Engineering and Technology
[SDV.IB]Life Sciences [q-bio]/Bioengineering
Anatomy
Research Article
Paper
Arthropoda
Equipment
Research and Analysis Methods
Filter paper
Anopheles
parasitic diseases
Parasitic Diseases
Animals
[SDV.IB] Life Sciences [q-bio]/Bioengineering
lcsh:R
Organisms
Biology and Life Sciences
Insect Bites and Stings
Feeding Behavior
Tropical Diseases
Anopheles gambiae
Invertebrates
Insect Vectors
Malaria
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
Species Interactions
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
[SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, 2017, 12 (8), pp.e0183238. ⟨10.1371/journal.pone.0183238⟩, PLoS ONE, Public Library of Science, 2017, 12 (8), pp.e0183238. ⟨10.1371/journal.pone.0183238⟩, PLoS ONE, Vol 12, Iss 8, p e0183238 (2017)
- Accession number :
- edsair.pmid.dedup....d5acb234ade78ce532fe71bca0468753