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A P-glycoprotein is linked to resistance to the Bacillus thuringiensis Cry3Aa toxin in a leaf beetle
- Source :
- Toxins, Toxins, MDPI, 2016, 8 (12), pp.1-12. ⟨10.3390/toxins8120362⟩, Toxins 12 (8), 1-12. (2016), Toxins, Vol 8, Iss 12, p 362 (2016)
- Publication Year :
- 2016
-
Abstract
- Chrysomela tremula is a polyvoltine oligophagous leaf beetle responsible for massive attacks on poplar trees. This beetle is an important model for understanding mechanisms of resistance to Bacillus thuringiensis (Bt) insecticidal toxins, because a resistant C. tremula strain has been found that can survive and reproduce on transgenic poplar trees expressing high levels of the Cry3Aa Bt toxin. Resistance to Cry3Aa in this strain is recessive and is controlled by a single autosomal locus. We used a larval midgut transcriptome for C. tremula to search for candidate resistance genes. We discovered a mutation in an ABC protein, member of the B subfamily homologous to P-glycoprotein, which is genetically linked to Cry3Aa resistance in C. tremula. Cultured insect cells heterologously expressing this ABC protein swell and lyse when incubated with Cry3Aa toxin. In light of previous findings in Lepidoptera implicating A subfamily ABC proteins as receptors for Cry2A toxins and C subfamily proteins as receptors for Cry1A and Cry1C toxins, this result suggests that ABC proteins may be targets of insecticidal three-domain Bt toxins in Coleoptera as well.
- Subjects :
- Male
ATP Binding Cassette Transporter, Subfamily B
coleoptera
ABC proteins
lcsh:Medicine
Toxicology
Bt resistance
Article
identification de gènes
Cell Line
Insecticide Resistance
protéine ABCA1
Hemolysin Proteins
Bt Cry3Aa toxin
Chrysomela tremula
leaf beetle
Bacterial Proteins
chrysomela tremulae
Animals
cry3a
toxin
Toxicologie
résistance aux bacteries
Bacillus thuringiensis Toxins
chrysomelidae
lcsh:R
fungi
chafers
bacillus thuringiensis
Endotoxins
toxine
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Female
Subjects
Details
- ISSN :
- 20726651
- Database :
- OpenAIRE
- Journal :
- Toxins, Toxins, MDPI, 2016, 8 (12), pp.1-12. ⟨10.3390/toxins8120362⟩, Toxins 12 (8), 1-12. (2016), Toxins, Vol 8, Iss 12, p 362 (2016)
- Accession number :
- edsair.pmid.dedup....e593900c94cb1a804d3e6ad39b3e7869