Fast fluorescence dynamics in non-ratiometric calcium indicators

Authors :: Gersbach, M.
Boiko, D. L.
Niclass, C.
Petersen, C.
Charbon, E.
Source :: Opt. Lett. 34 (2009) 362-364
Publication Year :: 2008
Abstract: A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.<br />Comment: 3 pages, 2 figures, figures revised, added chi-square goodness of fit