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Developing and Testing a Bayesian Analysis of Fluorescence Lifetime Measurements

Authors :
Kaye, Bryan
Foster, Peter J.
Yoo, Tae Yeon
Needleman, Daniel J.
Source :
PLoS ONE (2017), 12(1): e0169337
Publication Year :
2016

Abstract

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM), which relies on the reduction of fluorescence lifetime when a fluorophore is undergoing FRET. FLIM measurements take the form of histograms of photon arrival times, containing contributions from a mixed population of fluorophores both undergoing and not undergoing FRET, with the measured distribution being a mixture of exponentials of different lifetimes. Here, we present an analysis method based on Bayesian inference that rigorously takes into account several experimental complications. We test the precision and accuracy of our analysis on controlled experimental data and verify that we can faithfully extract model parameters, both in the low-photon and low-fraction regimes.<br />Comment: * These authors contributed equally to this work

Details

Database :
arXiv
Journal :
PLoS ONE (2017), 12(1): e0169337
Publication Type :
Report
Accession number :
edsarx.1607.03063
Document Type :
Working Paper
Full Text :
https://doi.org/10.1371/journal.pone.0169337