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Prediction of cellular burden with host-circuit models

Authors :
Nikolados, Evangelos-Marios
Weiße, Andrea Y.
Oyarzún, Diego A.
Publication Year :
2020

Abstract

Heterologous gene expression draws resources from host cells. These resources include vital components to sustain growth and replication, and the resulting cellular burden is a widely recognised bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host-circuit models to predict the impact of design parameters on both burden and circuit functionality. Our approach relies on a new generation of computational models for microbial growth that can flexibly accommodate resource bottlenecks encountered in gene circuit design. Adoption of this modelling paradigm can facilitate fast and robust design cycles in synthetic biology.

Details

Database :
arXiv
Publication Type :
Report
Accession number :
edsarx.2004.00995
Document Type :
Working Paper