Deep learning assisted SERS detection of prolines and hydroxylated prolines using nitrilotriacetic acid functionalized gold nanopillars

Proline (Pro) is one kind of proteinogenic amino acid and an important signaling molecule in the process of metabolism. Hydroxyproline (Hyp) is a product on Pro oxygen sensing post-translational modification (PTM), which is efficiently modulated tumor cells for angiogenesis. Distinguishing between Pro and Hyp is crucial for diagnosing connective tissue disorders, as elevated levels of Hyp can indicate abnormal collagen metabolism, often associated with diseases like osteogenesis imperfecta or fibrosis. However, there is a very small difference between molecular structures of Pro and Hyp, which is a big challenge for current detection technologies to distinguish them. For surface-enhanced Raman scattering (SERS) sensors, the similar molecule structure leads to similar Raman spectra that are difficult to distinguish. Furthermore, another problem is the weak affinity between amino acids sample and SERS-active substrates by physical adsorption. The selecting capturing of Pro and Hyp in the mixture of amino acids is not easy to achieve. In this work, we designed a new method for Pro and Hyp specifical detection and recognition by using gold nanopillars as the SERS substrate and combing nitrilotriacetic acid (NTA) with nickel (Ni) to form NTA-Ni structure as a specifical affinity agent. One side of NTA-Ni was attached to gold nanopillars through thiol binding. Another side captured the amino acids using reversible binding by receptor-ligand interaction between Ni and amino acids. Because of the different binding time with NTA-Ni and amino acids, the sensor can recognize Pro and Hyp from amino acids mixture. Then we used automatic peak assignment program for data analysis and machine learning model to distinguish between Pro and Hyp. The label-free SERS detection of amino acids PTM using gold nanopillars provides a potential method to further biomolecule detection and specifical capture.