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Development of a Real-time PCR test for porcine group A rotavirus diagnosis
- Source :
- Pesquisa Veterinária Brasileira, Vol 35, Iss 1, Pp 39-43 (2015)
- Publication Year :
- 2015
- Publisher :
- Colégio Brasileiro de Patologia Animal (CBPA), 2015.
-
Abstract
- Group A Rotavirus (RVA) is one of the most common causes of diarrhea in humans and several animal species. A SYBR-Green Real-Time polymerase chain reaction (PCR) was developed to diagnose RVA from porcine fecal samples, targeting amplification of a 137-bp fragment of nonstructural protein 5 (NSP5) gene using mRNA of bovine NADH-desidrogenase-5 as exogenous internal control. Sixty-five samples were tested (25 tested positive for conventional PCR and genetic sequencing). The overall agreement (kappa) was 0.843, indicating 'very good' concordance between tests, presenting 100% of relative sensitivity (25+ Real Time PCR/25+ Conventional PCR) and 87.5% of relative sensitivity (35- Real Time PCR/40- Conventional PCR). The results also demonstrated high intra- and inter-assay reproducibility (coefficient of variation ≤1.42%); thus, this method proved to be a fast and sensitive approach for the diagnosis of RVA in pigs.
- Subjects :
- Rotavírus
diagnóstico
PCR
PCR em Tempo Real
NSP5
suínos.
Veterinary medicine
SF600-1100
Subjects
Details
- Language :
- English, Portuguese
- ISSN :
- 16785150 and 0100736X
- Volume :
- 35
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Pesquisa Veterinária Brasileira
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0123f064db174d7daf108f3c1355659b
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/S0100-736X2015000100009