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Improvement of a synthetic live bacterial therapeutic for phenylketonuria with biosensor-enabled enzyme engineering

Authors :
Kristin J. Adolfsen
Isolde Callihan
Catherine E. Monahan
Per Jr. Greisen
James Spoonamore
Munira Momin
Lauren E. Fitch
Mary Joan Castillo
Lindong Weng
Lauren Renaud
Carl J. Weile
Jay H. Konieczka
Teodelinda Mirabella
Andres Abin-Fuentes
Adam G. Lawrence
Vincent M. Isabella
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

PKU patients have elevated phenylalanine levels which can result in neurological impairment. Here the authors utilize biosensor-based ultra-high-throughput screening to optimize PAL activity in a synthetic biotic platform for improved in vivo performance.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.026a2581af754d8aa8526f2be41ac1f4
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-26524-0