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KCNJ14 knockdown significantly inhibited the proliferation and migration of colorectal cells

Authors :
Bin Li
Ning Ge
Zhongping Pan
Chaofeng Hou
Kun Xie
Dongfang Wang
Junwei Liu
Jie Wan
Feihong Deng
Mengyi Li
Shuping Luo
Source :
BMC Medical Genomics, Vol 15, Iss 1, Pp 1-13 (2022)
Publication Year :
2022
Publisher :
BMC, 2022.

Abstract

Abstract Background This study attempted to verify the potential of KCNJ14 as a biomarker in colorectal cancer (CRC). Methods Data on transcriptomics and DNA methylation and the clinical information of CRC patients were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus databases. Biological information analysis methods were conducted to determine the role of KCNJ14 in the prognosis, diagnosis, immune cell infiltration, and regulation mechanism of CRC patients. The effect of KCNJ14 on the proliferation and migration of HCT116 and SW480 CRC cell lines was verified by in vitro experiments (MTT, colony-forming, wound healing, and transwell assays). Western blotting was performed to detect the effect of KCNJ14 on the levels of mTOR signalling pathway-related proteins. Results KCNJ14 expression was remarkably increased in CRC tissues and cell lines, which reduced the overall survival time of patients. KCNJ14 mRNA was negatively regulated by its methylation site cg17660703, which can also endanger the prognosis of patients with CRC. Functional enrichment analysis suggested that KCNJ14 is involved in the mTOR, NOD-like receptor, and VEGF signalling pathways. KCNJ14 expression was positively correlated with the number of CD4 + T cells and negatively correlated with that of CD8 + T cells in the immune microenvironment. KCNJ14 knockdown significantly reduced not only the proliferation and migration of CRC cell lines but also the levels of mTOR signalling pathway-related proteins. Conclusions This study not only increases the molecular understanding of KCNJ14 but also provides a potentially valuable biological target for the treatment of colorectal cancer.

Details

Language :
English
ISSN :
17558794
Volume :
15
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Medical Genomics
Publication Type :
Academic Journal
Accession number :
edsdoj.03d643f344f74d52b802af472f5ba821
Document Type :
article
Full Text :
https://doi.org/10.1186/s12920-022-01351-4