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Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid(光合细菌产生5-氨基乙酰丙酸(ALA)的研究)

Authors :
LIUXiu-yang(刘秀艳)
XUXiang-yang(徐向阳)
CHENWei-qing(陈蔚青)
Source :
Zhejiang Daxue xuebao. Lixue ban, Vol 29, Iss 3, Pp 336-340 (2002)
Publication Year :
2002
Publisher :
Zhejiang University Press, 2002.

Abstract

从36个光合细菌菌株中筛选出的7株紫色非硫红假单胞菌有产ALA能力,其中菌株99-28 ALA产量最高.用紫外线对菌株99-28进行诱变处理,筛选出产量比野生菌株高1倍多的菌株L-1.对影响菌株的生长和ALA产量的因子进行了探索.抑制剂加入时间和加入次数对ALA产量有显著影响.在最佳条件下(pH 7.5,培养对数生长期加入ALA脱水酶抑制剂乙酰丙酸30 mmol,ALA生物合成前体甘氨酸30 mmol和琥珀酸30 mmol,3 000 lx光照),菌株L-1的ALA产量可达22.15 mg/L.

Details

Language :
Chinese
ISSN :
10089497
Volume :
29
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Zhejiang Daxue xuebao. Lixue ban
Publication Type :
Academic Journal
Accession number :
edsdoj.0728658cbca485196051747f725962c
Document Type :
article