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Transcriptional profile of the rat cardiovascular system at single-cell resolution

Transcriptional profile of the rat cardiovascular system at single-cell resolution

Authors :
Alessandro Arduini
Stephen J. Fleming
Ling Xiao
Amelia W. Hall
Amer-Denis Akkad
Mark D. Chaffin
Kayla J. Bendinelli
Nathan R. Tucker
Irinna Papangeli
Helene Mantineo
Patricio Flores-Bringas
Mehrtash Babadi
Christian M. Stegmann
Guillermo García-Cardeña
Mark E. Lindsay
Carla Klattenhoff
Patrick T. Ellinor
Source :
Cell Reports, Vol 44, Iss 1, Pp 115091- (2025)
Publication Year :
2025
Publisher :
Elsevier, 2025.

Abstract

Summary: We sought to characterize cellular composition across the cardiovascular system of the healthy Wistar rat, an important model in preclinical cardiovascular research. We performed single-nucleus RNA sequencing (snRNA-seq) in 78 samples in 10 distinct regions, including the four chambers of the heart, ventricular septum, sinoatrial node, atrioventricular node, aorta, pulmonary artery, and pulmonary veins, which produced 505,835 nuclei. We identified 26 distinct cell types and additional subtypes, with different cellular composition across cardiac regions and tissue-specific transcription for each cell type. Several cell subtypes were region specific, including a subtype of vascular smooth muscle cells enriched in the large vasculature. We observed tissue-enriched cellular communication networks, including heightened Nppa-Npr1/2/3 signaling in the sinoatrial node. The existence of tissue-restricted cell types suggests regional regulation of cardiovascular physiology. Our detailed transcriptional characterization of each cell type offers the potential to identify novel therapeutic targets and improve preclinical models of cardiovascular disease.

Details

Language :
English
ISSN :
22111247
Volume :
44
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Cell Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.0767ac83a4d74870a6d56da8ae01844d
Document Type :
article
Full Text :
https://doi.org/10.1016/j.celrep.2024.115091