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Can bovine embryos be successfully transferred after 40 years of cryopreservation?

Can bovine embryos be successfully transferred after 40 years of cryopreservation?

Authors :
J. Camacho-Ronquillo
E. Posadas-Manzano
F. Utrera-Quintana
J. Hernández-Hernández
J. Gallegos-Sánchez
J. Cruz-Aviña
A. Villa-Mancera
Source :
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 75, Iss 5, Pp 909-913 (2023)
Publication Year :
2023
Publisher :
Universidade Federal de Minas Gerais, 2023.

Abstract

ABSTRACT Global advances in reproductive biotechnology have allowed for the transfer of embryos from donor females with high genetic merit to recipients using the cryopreservation technique, which preserves an embryo of excellent quality and viability, thereby achieving a feasible pregnancy rate. The objective of this study was to evaluate the quality and viability of Holstein embryos that have been cryopreserved for more than 40 years under glycerol freezing. The embryos were transferred to the recipient heifers using a non-surgical method. Two 17-month-old Holstein heifers (360 kg live weights) which were clinically healthy and reproductively active were used as the recipients. Two bovine embryos of Grade 1 quality were thawed and evaluated for their morphology. Of the two embryo transfers, one pregnancy was achieved, resulting in the birth of a calf. Therefore, embryos frozen in liquid nitrogen and glycerol as a cryopreservative for more than 40 years maintained their quality and viability to produce a live calf.

Details

Language :
English, Portuguese
ISSN :
16784162
Volume :
75
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Publication Type :
Academic Journal
Accession number :
edsdoj.0808d9f47704949960ebb4b1f4a55f9
Document Type :
article
Full Text :
https://doi.org/10.1590/1678-4162-13035