Comparison of the nutrient value, nonspecific immunity, and intestinal microflora of red swamp crayfish (Procambarus clarkii) in different culture modes

This study aimed to compare the nutritional composition, digestive enzyme activity, nonspecific immunity, and intestinal microflora of Procambarus clarkii under different culture modes: crayfish and crab coculture, pond intensive farmed culture (PF), rice and crayfish coculture (RC), cement pond culture (CP), and wild-caught. The muscle, hepatopancreas, and intestine of crayfish with a mean weight of 25.35 ± 4.23 g were collected and examined. The results showed that the nutritive value of amino acids and fatty acids in the muscle was higher in the RC group than in the other groups. Moreover, muscle crude protein contents were the highest in the CP group. Trypsin activities in the intestines were significantly higher in the PF and CP groups than in the other groups (P