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Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

Authors :
Evelyn Rabelo Andrade
Poul Maddox-Hyttel
Fernanda Da Cruz Landim-Alvarenga
José Roberto Viana Silva
Amauri Alcindo Alfieri
Marcelo Marcondes Seneda
José Ricardo Figueiredo
Ricardo Toniolli
Source :
Veterinary Medicine International, Vol 2011 (2011)
Publication Year :
2011
Publisher :
Hindawi Limited, 2011.

Abstract

The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

Subjects

Subjects :
Veterinary medicine
SF600-1100

Details

Language :
English
ISSN :
20420048
Volume :
2011
Database :
Directory of Open Access Journals
Journal :
Veterinary Medicine International
Publication Type :
Academic Journal
Accession number :
edsdoj.0aea447c253445b88498fba78d49d50f
Document Type :
article
Full Text :
https://doi.org/10.4061/2011/670987