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Successful Extension of Vascularized Composite Allograft Perfusion Cold Storage to 24 h in a Rat Hindlimb Transplant Model
- Source :
- Transplantation Direct, Vol 10, Iss 6, p e1623 (2024)
- Publication Year :
- 2024
- Publisher :
- Wolters Kluwer, 2024.
-
Abstract
- Background. Vascularized composite allograft transplantation is a treatment option for complex tissue injuries; however, ischemia reperfusion injury and high acute rejection rates remain a challenge. Hypothermic machine perfusion using acellular storage perfusate is a potential solution. This study evaluated the University of Wisconsin Kidney Preservation Solution-1 (KPS-1) compared with normal saline (NS) for preservation of donor rat hindlimbs subjected to 24 h of ex vivo perfusion cold storage. Methods. Hindlimbs were subjected to 24-h perfusion cold storage with heparinized KPS-1 (n = 6) or heparinized NS (n = 6). Flow, resistance, and pH were measured continuously. At the end of the 24-h period, tissue was collected for histological analysis of edema and apoptosis. Results. KPS-1 perfused limbs showed significantly less edema than the NS group, as evidenced by lower limb weight gain (P
Details
- Language :
- English
- ISSN :
- 23738731 and 00000000
- Volume :
- 10
- Issue :
- 6
- Database :
- Directory of Open Access Journals
- Journal :
- Transplantation Direct
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0cb3089453ff4d75963c5a6f190798af
- Document Type :
- article
- Full Text :
- https://doi.org/10.1097/TXD.0000000000001623