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Molecular Evaluation of the Impact of Nd:YAG Laser and Static Magnetic Field on Genomic DNA of Some Bacterial Isolates using RAPD-PCR

Authors :
Qanat Mahmood Atiyea
Fatima Mustafa Al-najar
Gulbahar F. Karim
Siham Sh. AL-Salihi
Source :
Journal of Pure and Applied Microbiology, Vol 16, Iss 3, Pp 2072-2082 (2022)
Publication Year :
2022
Publisher :
Journal of Pure and Applied Microbiology, 2022.

Abstract

Antimicrobial therapy is frequently associated with the emergence of resistant bacteria with a high rate of morbidity and mortality worldwide. The present study was aimed at investigating the impact of a neodymium-doped yttrium aluminum (Nd:YAG) laser, and a static magnetic field (SMF) on cellular growth and DNA alteration in some clinical bacterial isolates. Samples from cutaneous wounds were collected by sterile cotton swabs from three elderly women admitted to Tikrit Teaching Hospital, Tikrit City, Iraq. Isolation and identification of Streptococcus agalactiae, Staphylococcus aureus, and Pseudomonas aeruginosa were carried out using cultural characteristics, microscopy, and biochemical tests. Three broth cultures were prepared for each of the test isolates. The first broth culture served as untreated control, the second was exposed to an Nd:YAG laser and the third was exposed to SMF. Colony counting was done on all the samples. DNA was extracted from the test bacteria and used to perform the RAPD-PCR assay. In contrast to the untreated control, the results showed that Nd:YAG laser radiation was more effective than SMF at inhibiting the cellular growth of the test isolates. Also, the radiation caused DNA alteration, which was established by decreased microbial growth, as well as the development of new bands and the loss of original bands. According to the findings of this study, the Nd:YAG laser is a promising technique for influencing the healing of infected cutaneous wounds. RAPD-PCR is also a useful biomarker assay for assessing the biological impact of laser radiation and SMF on bacteria.

Details

Language :
English
ISSN :
09737510 and 2581690X
Volume :
16
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Journal of Pure and Applied Microbiology
Publication Type :
Academic Journal
Accession number :
edsdoj.0d8427dfeca84f92857d30cc2e4ca24d
Document Type :
article
Full Text :
https://doi.org/10.22207/JPAM.16.3.62