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Effects of hippocampal PGC-1α on depression-like behaviors and number of astrocytes in hippocampal subregions in mice
- Source :
- Di-san junyi daxue xuebao, Vol 44, Iss 10, Pp 975-983 (2022)
- Publication Year :
- 2022
- Publisher :
- Editorial Office of Journal of Third Military Medical University, 2022.
-
Abstract
- Objective To investigate the effects of targeted silence/overexpression of PGC-1α in the hippocampus on depression-like behaviors in mice and on the number of glial fibrillary acidic protein positive (GFAP+) astrocytes in the subregions of the hippocampus. Methods After adaptive feeding and sucrose preference baseline adjustment, 32 male C57BL/6 mice aged 6~8 weeks were randomly divided into empty virus group (n=14), PGC-1α silencing group (n=11) and PGC-1α overexpression group (n=7). The latter 2 groups were injected with adeno-associated virus (AAV)-mediated genes silencing or overexpressing PGC-1α in the hippocampus, respectively, with aid of stereotactic injection technique. Sucrose preference test (SPT) and forced swimming test (FST) were performed to assess the depression-like behaviors, and the number of GFAP+ astrocytes in each hippocampal subregion was accurately quantified in 3 dimensions by immunohistochemistry and stereology. Results As compared with the empty virus group, the PGC-1α silencing group had significantly decreased percentage of sucrose preference (P < 0.01), prolonged immobility time in FST (P < 0.05), and larger number of GFAP+ astrocytes in both CA1 and DG subregions(P < 0.05). However, the immobility time in FST was remarkably shortened in the PGC-1α overexpression group (P < 0.01), with the number of GFAP+ astrocytes in CA1 and DG subregions diminished (P < 0.05). There was no significant difference in the percentage of sucrose preference between the empty virus group and PGC-1α overexpression group, and silencing or overexpressing PGC-1α showed no obvious impact on the number of astrocytes in CA3 subregion. Conclusion PGC-1α is closely associated with the depression-like behaviors and participates in regulating the number of GFAP+ astrocytes in CA1 and DG subregions in mice.
Details
- Language :
- Chinese
- ISSN :
- 20970927 and 10005404
- Volume :
- 44
- Issue :
- 10
- Database :
- Directory of Open Access Journals
- Journal :
- Di-san junyi daxue xuebao
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.0dadb23cba94887a5046cdc72944604
- Document Type :
- article
- Full Text :
- https://doi.org/10.16016/j.2097-0927.202110068