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Comparison of quantification methods for an endoscope lumen biofilm model

Authors :
Bruno Haas
Sarah James
Albert E. Parker
Marie-Claude Gagnon
Noémie Goulet
Philippe Labrie
Source :
Biofilm, Vol 6, Iss , Pp 100163- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Biofilm has been implicated in multi-drug resistant organism outbreaks following endoscopic procedures. Automated Endoscope Reprocessors (AER) are devices validated to clean and disinfect endoscopes per applicable standards. The ISO 15883 part 4 standard guides performance testing validation of AERs, including cleaning performance using a biofilm test soil. The standard recommends assessment of biofilm reduction using protein or carbohydrate quantification methods. The aim of this study was to assess the suitability of various quantification methods using the ISO biofilm model.The ISO 15883 part 5 biofilm test soil method was used to grow biofilm within lumens representative of endoscopes channels. The biofilm was then quantified using five methods: Crystal Violet (CV), Colony Forming Units (CFU), Total Organic Carbon (TOC), protein assay with Orthophtalaldehyde (OPA), and protein assay by micro bicinchoninic acid (μBCA). The five methods were statistically analyzed for their ability to assess biofilm reduction on samples accurately and precisely. In addition, the quantification methods were compared to demonstrate statistical equivalency, and thus their suitability for assessing biofilm cleaning performance testing of AERs.

Details

Language :
English
ISSN :
25902075
Volume :
6
Issue :
100163-
Database :
Directory of Open Access Journals
Journal :
Biofilm
Publication Type :
Academic Journal
Accession number :
edsdoj.0fb052cfe155449a94c89c289b6a88a3
Document Type :
article
Full Text :
https://doi.org/10.1016/j.bioflm.2023.100163