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Immunohistochemical analysis of adipokine and adipokine receptor expression in the breast tumor microenvironment: associations of lower leptin receptor expression with estrogen receptor-negative status and triple-negative subtype

Authors :
Adana A. M. Llanos
Yong Lin
Wenjin Chen
Song Yao
Jorden Norin
Marina A. Chekmareva
Coral Omene
Lei Cong
Angela R. Omilian
Thaer Khoury
Chi-Chen Hong
Shridar Ganesan
David J. Foran
Michael Higgins
Christine B. Ambrosone
Elisa V. Bandera
Kitaw Demissie
Source :
Breast Cancer Research, Vol 22, Iss 1, Pp 1-16 (2020)
Publication Year :
2020
Publisher :
BMC, 2020.

Abstract

Abstract Background The molecular mechanisms underlying the association between increased adiposity and aggressive breast cancer phenotypes remain unclear, but likely involve the adipokines, leptin (LEP) and adiponectin (ADIPOQ), and their receptors (LEPR, ADIPOR1, ADIPOR2). Methods We used immunohistochemistry (IHC) to assess LEP, LEPR, ADIPOQ, ADIPOR1, and ADIPOR2 expression in breast tumor tissue microarrays among a sample of 720 women recently diagnosed with breast cancer (540 of whom self-identified as Black). We scored IHC expression quantitatively, using digital pathology analysis. We abstracted data on tumor grade, tumor size, tumor stage, lymph node status, Ki67, estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) from pathology records, and used ER, PR, and HER2 expression data to classify breast cancer subtype. We used multivariable mixed effects models to estimate associations of IHC expression with tumor clinicopathology, in the overall sample and separately among Blacks. Results Larger proportions of Black than White women were overweight or obese and had more aggressive tumor features. Older age, Black race, postmenopausal status, and higher body mass index were associated with higher LEPR IHC expression. In multivariable models, lower LEPR IHC expression was associated with ER-negative status and triple-negative subtype (P

Details

Language :
English
ISSN :
1465542X
Volume :
22
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Breast Cancer Research
Publication Type :
Academic Journal
Accession number :
edsdoj.1380d7cb36394a49a6c3cddcb0b2a0d1
Document Type :
article
Full Text :
https://doi.org/10.1186/s13058-020-1256-3