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The Novel Lipopeptide Poaeamide of the Endophyte Pseudomonas poae RE*1-1-14 Is Involved in Pathogen Suppression and Root Colonization

Authors :
Christin Zachow
Ghazaleh Jahanshah
Irene de Bruijn
Chunxu Song
Federica Ianni
Zoltán Pataj
Heike Gerhardt
Isabelle Pianet
Michael Lämmerhofer
Gabriele Berg
Harald Gross
Jos M. Raaijmakers
Source :
Molecular Plant-Microbe Interactions, Vol 28, Iss 7, Pp 800-810 (2015)
Publication Year :
2015
Publisher :
The American Phytopathological Society, 2015.

Abstract

Endophytic Pseudomonas poae strain RE*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen Rhizoctonia solani both in vitro and in the field. To identify genes involved in its biocontrol activity, RE*1-1-14 random mutagenesis and sequencing led to the identification of a nonribosomal peptide synthetase (NRPS) gene cluster predicted to encode a lipopeptide (LP) with a 10-amino-acid peptide moiety. The two unlinked gene clusters consisted of three NRPS genes, designated poaA (cluster 1) and poaB and poaC (cluster 2), spanning approximately 33.7 kb. In silico analysis followed by chemical analyses revealed that the encoded LP, designated poaeamide, is a structurally new member of the orfamide family. Poaeamide inhibited mycelial growth of R. solani and different oomycetes, including Phytophthora capsici, P. infestans, and Pythium ultimum. The novel LP was shown to be essential for swarming motility of strain RE*1-1-14 and had an impact on root colonization of sugar beet seedlings The poaeamide-deficient mutant colonized the rhizosphere and upper plant cortex at higher densities and with more scattered colonization patterns than the wild type. Collectively, these results indicate that Pseudomonas poae RE*1-1-14 produces a structurally new LP that is relevant for its antagonistic activity against soilborne plant pathogens and for colonization of sugar beet roots.

Subjects

Subjects :
Microbiology
QR1-502
Botany
QK1-989

Details

Language :
English
ISSN :
19437706 and 08940282
Volume :
28
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Molecular Plant-Microbe Interactions
Publication Type :
Academic Journal
Accession number :
edsdoj.15bf22afa14546fdb93db5b5ec5116c4
Document Type :
article
Full Text :
https://doi.org/10.1094/MPMI-12-14-0406-R