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Alpha-Lipoic Acid Ameliorates Impaired Steroidogenesis in Human Granulosa Cells Induced by Advanced Glycation End-Products

Authors :
Zahra Derakhshan
Soghra Bahmanpour
Mohammad Hossein Nasr-Esfahani
Fatemeh Masjedi
Maryam Mirani
Mahintaj Dara
Seyed Mohammad Bagher Tabei
Source :
Iranian Journal of Medical Sciences, Vol 49, Iss 8, Pp 515-527 (2024)
Publication Year :
2024
Publisher :
Shiraz University of Medical Sciences, 2024.

Abstract

Background: Ovarian granulosa cells (GCs) are essential for follicular development. Ovarian advanced glycation end-products (AGEs) accumulation is related to GCs dysfunction. Alpha-lipoic acid (ALA) illustrates therapeutic capabilities for infertility-related disorders. Therefore, this study assessed the effects of ALA on AGEs-induced GCs hormonal dysfunction.Methods: The study was conducted from October 2021 to September 2022 at the Department of Medical Genetics, Shiraz University of Medical Sciences. Isolated GCs (n=50) were divided into control, human glycated albumin (HGA), HGA+ALA, and ALA treatments. Steroidogenic enzymes and AGE receptor (RAGE) genes were assessed by qRT-PCR. Steroid hormones and RAGE protein were evaluated using ELISA and Western blotting. Data were analyzed using GraphPad Prism software (ver. 9), and P

Details

Language :
English
ISSN :
02530716 and 17353688
Volume :
49
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Iranian Journal of Medical Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.16ecfbc34ed44c658d2a461ff941440f
Document Type :
article
Full Text :
https://doi.org/10.30476/ijms.2023.99512.3168