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EFFICACY ASSESSMENT OF CRYO STORAGE OF DONOR HEARTS BY IMAGEJ BASED IMAGE ANALYSIS

Authors :
Bao-quan Lin
Sheng-sheng Yang
Zhi-yong Zeng
Cong-wen Zhuang
Source :
Российский кардиологический журнал, Vol 0, Iss 1-ENG, Pp 45-48 (2014)
Publication Year :
2014
Publisher :
«FIRMA «SILICEA» LLC, 2014.

Abstract

Aim. Donor organ injury during cold preservation before transplantation negatively impacts graft survival. The current study was to examine available evidences for the efficacy of different cold storage solutions that are used to preserve donor hearts in vitro prior to orthotopic transplantation.Material and methods. A systematic search of full-length articles published from 1980 to August 2012 was performed in PubMed and Google Scholar. Detailed searches were also made for availability of any sourceware for histopathology images of endomyocardial biopsies of stored hearts.Results. Not even a single controlled trial has been published relating to this topic. However, we assessed all available literature pertaining to this topic, and performed original, simple yet innovative analyses using ImageJ, a Java based image analyses program, to show the tremendous power to objectively examine the efficacy of the storage solution. Our analysis suggest that ImageJ may be conveniently used to obtain evidences (or lack of it) of ischemic injury of donor hearts during cold storage.Conclusions. Even the UNOS database does not provide histopathological evidences of cardiac biopsies of orthotopically transplanted hearts. We, however, make the case of the need for image analyses and making availability of images to allow establishing evidence of the usefulness of these storage solutions. We recommend obtaining endomyocardial biopsy prior to orthotopic transplantation and create a registry of H&E stained slides. This is the only step that will direct us towards evidence based care of such highly critical patients who need the equally challenging surgical intervention of cardiac transplantation.

Details

Language :
Russian
ISSN :
15604071 and 26187620
Issue :
1-ENG
Database :
Directory of Open Access Journals
Journal :
Российский кардиологический журнал
Publication Type :
Academic Journal
Accession number :
edsdoj.17895e0c1c948cd8972f64a0b3968bd
Document Type :
article
Full Text :
https://doi.org/10.15829/1560-4071-2014-1-ENG-45-48