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Epidemiology and molecular identification of mixed yeast isolates in Malaysia- A way forward
- Source :
- Current Medical Mycology, Vol 8, Iss 3, Pp 35-38 (2022)
- Publication Year :
- 2022
- Publisher :
- Mazandaran University of Medical Sciences, 2022.
-
Abstract
- Background and Purpose: Invasive candidiasis is one of the most common systemicmycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeastculture data.Materials and Methods: Yeast isolates were collected in Sultan Abdul Halim Hospital,North Malaysia, from October 2020 to October 2021. Chromogenic Candida differentialagar media and PCR-RFLP were used to identify yeast species.Results: A total of 206 yeast isolates were collected from different body sites of patients.The majority of the yeast isolates (n=104) were obtained from the urine. Other isolates were extracted from blood (n=52), vaginal swabs (n=45), ear discharge (n=2), tracheal aspirate (n=2), tissue (n=2), skin (n=1), nail (n=1), sputum (n=1), and cerebrospinal fluid (n=1). In total, 200 yeast samples were identified as single species, and six isolates were a mixture of Candida species.Conclusion: Malaysia lacks accurate epidemiological data on mixed yeast infections.We identified all samples to the species level, including mixed yeast cultures, using theMspI enzyme and PCR-RFLP.
Details
- Language :
- English
- ISSN :
- 24233439 and 24233420
- Volume :
- 8
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Current Medical Mycology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.18c264358331463198bc724da0c21917
- Document Type :
- article
- Full Text :
- https://doi.org/10.18502/cmm.8.3.11209