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Isolation of antibacterial response genes from the dung beetle Copris tripartitus (Coleoptera: Scarabaeidae) immunized with Escherichia coli
- Source :
- European Journal of Entomology, Vol 105, Iss 2, Pp 355-359 (2008)
- Publication Year :
- 2008
- Publisher :
- Institute of Entomology, Biology Centre, Czech Academy of Science, 2008.
-
Abstract
- Dot blot hybridization of total RNA from normal and bacteria-injected larvae of the dung beetle Copris tripartitus identified 13 cDNA clones that seem to be associated with the immune response. A cDNA encoding a dung beetle homolog of the heat shock protein HSP70 (CTHSP70) was characterized by full-length sequencing and expression pattern analysis. CTHSP70 was upregulated 4 h after bacterial injection, reached maximum level after 8 h, and declined gradually after 16 h. Our data suggest that CTHSP70 and another 12 genes are involved in the anti-microbial defense that is particularly important for dung beetle larvae living in pathogen-rich conditions.
- Subjects :
- antibacterial gene
dung beetle
hsp70
Zoology
QL1-991
Subjects
Details
- Language :
- English
- ISSN :
- 12105759 and 18028829
- Volume :
- 105
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- European Journal of Entomology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.19a99d01799a461eb0b96a6e22072eba
- Document Type :
- article
- Full Text :
- https://doi.org/10.14411/eje.2008.042