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Tuberculosis diagnosis: algorithm that May discriminate latent from active tuberculosis

Tuberculosis diagnosis: algorithm that May discriminate latent from active tuberculosis

Authors :
Elvis Ndukong Ndzi
Celine Nguefeu Nkenfou
Eric Walter Yone Pefura
Linda Chapdeleine Mouafo Mekue
Elise Guiedem
Carine Nkenfou Nguefeu
Marie Nicole Ngoufack
Elise Elong
Laeticia Grace Yatchou
Alexis Ndjolo
Jules-Roger Kuiate
Source :
Heliyon, Vol 5, Iss 10, Pp e02559- (2019)
Publication Year :
2019
Publisher :
Elsevier, 2019.

Abstract

Diagnosis of tuberculosis still faces a lot of challenges and is one of the priorities in the field of tuberculosis management. Deciphering the complex tuberculosis pathogenicity network could provide biomarkers for diagnosis. We discussed the distribution of HLA-B17, -DQB and -DRB together with QuantiFERON test results in tuberculosis infection. A case control study was done during which a total of 337 subjects were enrolled comprising 227 active tuberculosis (ATB), 46 latent tuberculosis infection (LTBI) and 64 healthy controls (HC). Sequence-specific primer polymerase chain reaction and immune epitope database were used to genotype samples and determine the epitope binding ability of the over-represented alleles respectively. QuantiFERON test was done according to manufacturer's instructions. The peptides HLA-B*5801 and HLA-DRB1*12 and the peptides HLA-B*5802 and HLA-DQB1*03 were found to be associated with latent tuberculosis while the haplotypes DRB1*10-DQB1*02 and DRB1*13-DQB1*06 were found to be associated with active tuberculosis (All p-values≤0.05). The association of HLA-B*5801 and HLA-B*5802 with latent tuberculosis was linked to their ability to bind or not mycobacterial antigens. DRB1*10-DQB1*02 haplotype was found to be over-represented in LTBI compared to ATB (p-value = 0.0015) while DRB1*13-DQB1*06 was found to be under-represented in LTBI compared to ATB (p-value = 0.0335). The DRB1*10-DQB1*02 haplotype was only found in the LTBI when compared with the ATB group. The present study suggests the following algorithm to discriminate LTBI from ATB: QuantiFERON+ and DRB1*10-DQB1*02 haplotype + may indicate LTBI; QuantiFERON+ and DRB1*10-DQB1*02 haplotype - may indicate ATB.

Details

Language :
English
ISSN :
24058440
Volume :
5
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Heliyon
Publication Type :
Academic Journal
Accession number :
edsdoj.19aa82fe8d334b1a8228a686cca2014e
Document Type :
article
Full Text :
https://doi.org/10.1016/j.heliyon.2019.e02559