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Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity
- Source :
- Virology Journal, Vol 7, Iss 1, p 294 (2010)
- Publication Year :
- 2010
- Publisher :
- BMC, 2010.
-
Abstract
- Abstract Background The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA. Results Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101Δ(A+C) was constructed. The present experiments indicated that GX0101Δ(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bi-directional promoter between 1.8-kb mRNA and pp38 genes of MDV. Conclusion These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV.
- Subjects :
- Infectious and parasitic diseases
RC109-216
Subjects
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 7
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Virology Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.1a707e1e0d44a748ae3c258fb931d19
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/1743-422X-7-294