Back to Search Start Over

Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential

Authors :
Ana Cvetko
Domagoj Kifer
Olga Gornik
Lucija Klarić
Elizabeth Visser
Gordan Lauc
James F. Wilson
Tamara Štambuk
Source :
Biomedicines, Vol 8, Iss 10, p 410 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology. Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis. We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls. Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10−3) and abundance of high-mannose structures (p = 1.48 × 10−2) were the most prominently altered IgG glycosylation traits. Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10−2–4.28 × 10−2). Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models. Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential. N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.

Details

Language :
English
ISSN :
22279059
Volume :
8
Issue :
10
Database :
Directory of Open Access Journals
Journal :
Biomedicines
Publication Type :
Academic Journal
Accession number :
edsdoj.1af52a954f4ace8dc0b5a8713d49e6
Document Type :
article
Full Text :
https://doi.org/10.3390/biomedicines8100410