Vector transmission of lime witches\' broom Phytoplasma to Mexican lime seedlings under greenhouse condition

Despite successful lime witches'-broom (LWB) phytoplasma transmission by Hishimonus phycitis to the lime trees and Bakraee seedlings, there is no published document regarding LWB transmission by H. phycitis to lime seedlings. To study the possibility of vector-based transmission to lime seedlings, the feral leafhoppers were collected in LWB-infected lime orchards and caged on one-year old Mexican lime seedlings. Six months after inoculation, 50% of inoculated seedlings showed typical symptoms of LWB and were strongly positive in PCR assays. To our knowledge, this is the first report of transmission of Ca. P. aurantifolia to Mexican lime seedlings by H. phycitis under greenhouse condition.