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Protocol for the derivation of primary cancer stem cell lines from human ependymal tumors
- Source :
- STAR Protocols, Vol 5, Iss 3, Pp 103260- (2024)
- Publication Year :
- 2024
- Publisher :
- Elsevier, 2024.
-
Abstract
- Summary: Cancer stem cells (CSCs) established from surgical biopsies closely mimic the human context and can be used to investigate disease mechanisms, genetic fitness, and therapeutic evaluation. Here, we present a protocol for the derivation of primary patient-derived CSC lines from ependymal tumors. We describe the necessary steps, from surgical intervention and biopsy to the dissociation of ependymomas to derive cultures. We then detail procedures for cell line propagation and define the characteristics of these primary cancer cell lines.For complete details on the use and execution of this protocol, please refer to Michealraj et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
- Subjects :
- Cell Biology
Cancer
Stem Cells
Cell Differentiation
Science (General)
Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 26661667
- Volume :
- 5
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- STAR Protocols
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.1bc5101bac0443238e48891b18b50ee4
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.xpro.2024.103260