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Calcium-mediated actin reset (CaAR) mediates acute cell adaptations

Authors :
Pauline Wales
Christian E Schuberth
Roland Aufschnaiter
Johannes Fels
Ireth García-Aguilar
Annette Janning
Christopher P Dlugos
Marco Schäfer-Herte
Christoph Klingner
Mike Wälte
Julian Kuhlmann
Ekaterina Menis
Laura Hockaday Kang
Kerstin C Maier
Wenya Hou
Antonella Russo
Henry N Higgs
Hermann Pavenstädt
Thomas Vogl
Johannes Roth
Britta Qualmann
Michael M Kessels
Dietmar E Martin
Bela Mulder
Roland Wedlich-Söldner
Source :
eLife, Vol 5 (2016)
Publication Year :
2016
Publisher :
eLife Sciences Publications Ltd, 2016.

Abstract

Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased intracellular calcium levels. Within seconds of calcium influx, the formin INF2 stimulates filament polymerization at the endoplasmic reticulum (ER), while cortical actin is disassembled. The reaction is then reversed within a few minutes. This Calcium-mediated actin reset (CaAR) occurs in a wide range of mammalian cell types and in response to many physiological cues. CaAR leads to transient immobilization of organelles, drives reorganization of actin during cell cortex repair, cell spreading and wound healing, and induces long-lasting changes in gene expression. Our findings suggest that CaAR acts as fundamental facilitator of cellular adaptations in response to acute signals and stress.

Details

Language :
English
ISSN :
2050084X
Volume :
5
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.1c44cc24da5646788f05eaf82ed3042d
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.19850