Proteomics analysis of exosome in mice with Candida albicans infection for biomarker

Objective To analyze serum exosome using proteomic in mice with Candida albicans infection and to find out marker. Methods Mice model of Candida albicans infection was established by injection of Candida albicans liquid through tail vein. Exosome in serum were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Mice models of both Staphylococcus aureus infection and Escherichia coli infection were established to compare the protein expression in exosome among three groups of infection. Results A total of 4 055 peptides and 418 proteins were identified. With the ratio 2.0 times between Candida albicans infection group and normal control group and with P