Study on the metabolite difference of tricolor quinoa from different habitats based on GC-MS

In order to study the effects of different habitats on the development of quinoa, taking quinoa seeds from different habitats as research objects, significant differences in metabolites and related metabolic pathways are screened. The metabolic profiles of the seeds of black quinoa, red quinoa and white quinoa from Dulan County, Qinghai Province and Yanyuan County, Sichuan Province are analyzed through non-targeted metabonomics analysis with GC-MS platform. The original data are pretreated by XCMS online platform for subsequent multivariate statistical analysis. The results show that the metabolic characteristics of black, red and white seeds in Dulan county and Yanyuan County are different. Among them, the difference between red and white seeds from the two counties is larger, while the difference of the black quinoa seeds is the smallest. There are 570 characteristic peaks in red seeds which have significant differences, and the up-regulated substances are less than the down-regulated. The difference between black quinoa from two habitats is the smallest, and the up- and down-regulated trends are like that of red quinoa. There is secondly significant difference between the white seeds from the two habitats, and the up- and down-regulated trends are the opposite of that of the red and black quinoas, and 486 characteristic peaks are detected, with more up-regulated substances than down-regulated. Different habitats have great impact on quinoa. Black and red quinoas are more suitable to be planted in Dulan County, Qinghai Province, while white quinoa is more suitable to be planted in Yanyuan County, Sichuan Province. The conclusions could guide the cultivation and breeding of quinoa in Dulan county and Yanyuan County, and provide theoretical basis for further study of the stress resistance mechanism of quinoa.