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Rapid detection of ceftriaxone-resistant Salmonella by matrix-assisted laser desorption–ionization time-of-flight mass spectrometry combined with the ratio of optical density
- Source :
- Annals of Clinical Microbiology and Antimicrobials, Vol 23, Iss 1, Pp 1-9 (2024)
- Publication Year :
- 2024
- Publisher :
- BMC, 2024.
-
Abstract
- Abstract Background The increased resistance rate of Salmonella to third-generation cephalosporins represented by ceftriaxone (CRO) may result in the failure of the empirical use of third-generation cephalosporins for the treatment of Salmonella infection in children. The present study was conducted to evaluate a novel method for the rapid detection of CRO-resistant Salmonella (CRS). Methods We introduced the concept of the ratio of optical density (ROD) with and without CRO and combined it with matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) to establish a new protocol for the rapid detection of CRS. Results The optimal incubation time and CRO concentration determined by the model strain test were 2 h and 8 µg/ml, respectively. We then conducted confirmatory tests on 120 clinical strains. According to the receiver operating characteristic curve analysis, the ROD cutoff value for distinguishing CRS and non-CRS strains was 0.818 [area under the curve: 1.000; 95% confidence interval: 0.970–1.000; sensitivity: 100.00%; specificity: 100%; P
Details
- Language :
- English
- ISSN :
- 14760711
- Volume :
- 23
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Annals of Clinical Microbiology and Antimicrobials
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.1e36ae677346491080a2546068d2fa54
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s12941-024-00729-9