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Identification and purification of immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine (Leishvacin®)

Authors :
Sandra Regina Afonso Cardoso
João Carlos França da Silva
Roberto Teodoro da Costa
Wilson Mayrink
Maria Norma Melo
Marilene Suzan Marques Michalick
Ibrahim Afrânio Willi Liu
Ricardo Toshio Fujiwara
Evaldo Nascimento
Source :
Revista da Sociedade Brasileira de Medicina Tropical, Vol 36, Iss 2, Pp 193-199 (2003)
Publication Year :
2003
Publisher :
Sociedade Brasileira de Medicina Tropical (SBMT), 2003.

Abstract

Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin®), produced by Biobrás (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30µg of each protein at 15-day intervals combined with 250µg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-g induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.

Details

Language :
English
ISSN :
16789849
Volume :
36
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Revista da Sociedade Brasileira de Medicina Tropical
Publication Type :
Academic Journal
Accession number :
edsdoj.1f7498b79b704632b4ea1115ec6f0fbd
Document Type :
article