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Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti-Porphyromonas gingivalis [version 4; peer review: 2 approved]

Authors :
Endang Winiati Bachtiar
Citra F. Putri
Retno D. Soejoedono
Boy M. Bachtiar
Source :
F1000Research, Vol 9 (2021)
Publication Year :
2021
Publisher :
F1000 Research Ltd, 2021.

Abstract

Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti-P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti-P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and iNOS2 of neuron cells.

Subjects

Subjects :
Medicine
Science

Details

Language :
English
ISSN :
20461402
Volume :
9
Database :
Directory of Open Access Journals
Journal :
F1000Research
Publication Type :
Academic Journal
Accession number :
edsdoj.1fdd475c8c4c4bcba774529180851e93
Document Type :
article
Full Text :
https://doi.org/10.12688/f1000research.26749.4